Figure 2.

Fluorescence anisotropy of DPH in HDL, DPPC, DPPC:Chol:lysoPC and DPPC:CE:lysoPC rHDLs as a function of temperature. The inflection point of the plot gives the phase transition temperature of high-density lipoproteins (HDL) and rHDLs. Insert shows the phase transition temperatures. DPH was excited at 360 nm and fluorescence anisotropy was measured at 428 nm. All measurements were carried out in TEN buffer pH 8. Concentration of HDL and rHDL was kept constant at apoA-I 2 µM for all measurements. Data points shown are means ± S.D. of at least three independent measurements. DPH: 1,6-diphenyl-1,3,5-hexatriene.