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. 2020 Sep 23;12(10):2734. doi: 10.3390/cancers12102734

Figure 5.

Figure 5

Involvement of aldehyde dehydrogenase (ALDH) activity in the sphere formation capacity of HDAC9-expressing undifferentiated HCC cells (a) Quantitative PCR of stemness-related genes Oct4, Bmi1, ALDH1A1, and ALDH1A3 in HLE (left) and HLF (right) cells treated with si-Control and si-HDAC9. The graphs are expressed as the relative expression to those of the si-Control group. UD; undetectable, ** p < 0.01 vs. si-Control. (b) Quantitative PCR of stemness-related genes in HLE (left) and HLF (right) cells treated with DMSO and BRD4354 (5 and 10 μM). Quantitative PCR analysis was performed at n = 4. * p < 0.05, ** p < 0.01 vs. DMSO group. (c) Cell proliferation assays of HLE (left) and HLF (right) cells treated with 0, 0.2, 0.5, and 1.0 μM of disulfiram (DSF) for 3 days. Cell viability was determined by WST assay and expressed as the relative amount of viable cells compared to day 0. (d) Spheres assay of HLE and HLF cells treated with 0, 0.2, and 0.5 μM of DSF for 7 days. The diameter of the sphere was measured in five representative spheres of each group. * p < 0.05, ** p < 0.01 vs. vehicle control.