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. 2020 Sep 23;12(10):1059. doi: 10.3390/v12101059

Figure 3.

Figure 3

AKT1 knockout (KO)and AKT2 KO 293T cell lines resist LGTV-induced a cytopathic effect (CPE). (a) Validation of CRISPR-mediated AKT1 and AKT2 knockouts in 293T cell lines with Western blotting analysis. (b) Stained monolayers comparing the susceptibility of AKT knock out cells to apoptosis. AKT1 KO, AKT2 KO, and WT 293T cells were infected with LGTV at an MOI of 0.1 and imaged at 168 hpi. Images were taken with a 10× objective. (c) Quantification of surviving cells at 168 hpi. * p < 0.05 (one-way ANOVA followed by Tukey’s multiple comparison test). Error bars indicate standard deviation from four independent experiments.