Figure 5.

LGTV-infected AKT knockout cells enter viral persistence without substantial alteration in viral replication. AKT1 KO, AKT2 KO, and WT 293T cells were infected with LGTV at an MOI of 0.1. (a) Viral titers of acute and persistently infected AKT KO and WT cell lines. Error bars indicate standard deviation from three independent experiments. (b) Immunofluorescence analysis evaluating expression of the viral E protein (red), a proxy for infection, at 168 hpi. Nuclei were counterstained with DAPI (blue). Images were taken with a 40× objective.