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. 2020 Sep 23;9(10):777. doi: 10.3390/pathogens9100777

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Colloidal Coomassie-stained 4–20% SDS-PAGE analysis of native and heated (for 60 min at 100 °C) crude (CR) antigen of A. simplex. (a). Western blot (WB) reactivity of anti-A. simplex rabbit IgG antibodies with native and heated (for 60 min at 100 °C) CR antigens of A. simplex. (b). Molecular weight (Mw) estimations in kilodaltons (kDa), densitometric plots of SDS-PAGE and WB profiles were performed using the Bio-1D software (Vilber Lourmat, ver. 15.07, Marne-la-Vallée, France). Green lines in the plots show profiles of the native antigen, while red lines show profiles of the heated antigen. Pos. -strips incubated with hyperimmune serum from a rabbit immunised with native A. simplex CR antigen; Neg. -strips incubated with rabbit pre-immune serum.