Table 1.

Overall densitometric measurements of SDS-PAGE and Western Blot (WB) profiles of native and heated (for 60 min at 100 °C) CR antigens of A. simplex. Values were calculated using the ImageJ software (National Institutes of Healths, ver. 1.53a, Bethesda, MD, USA).

	Mean Gray Value 5	SD of the Gray Values 6	Min Gray Value 7	Max Gray Value 8	Integrated Density 9
SDS-PAGE Native 1	184.8	42	56	255	50,513,856
SDS-PAGE Heated 2	184.93	35.23	102	254	49,073,619
WB Native 3	203.76	34.69	74	253	55,856,228
WB Heated 4	204	29.27	131	253	55,923,273

Densitometric measurements of SDS-PAGE profiles of native ¹ and heated ² antigens of A. simplex; densitometric measurements of WB profiles of native ³ and heated ⁴ antigens of A. simplex; mean gray value is the sum of the gray values of all the pixels in the profile divided by the number of pixels ⁵; standard deviation (SD) of the gray values used to generate the mean gray value ⁶; minimum ⁷ and maximum ⁸ gray values within the profile; integrated density is the sum of the values of the pixels in the profile ⁹.