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Limit of detection and precision of chemiluminescence immunoassays.
| Validation Parameter | CL-S-ELISA 1 | CL-C-ELISA 1 |
|---|---|---|
| Limit of detection (ng/mL) | 0.5 | 5 |
| Intra-assay precision (CV% 2) | 1.5–4.3 | 5.1–7.5 |
| Inter-assay precision (CV%) | 2.7–7.7 | 6.2–13.5 |
1 The limit of detection, intra-assay precision, and inter-assay precision were calculated using trout samples non-spiked and spiked with A. simplex antigen, which were subsequently heated for 60 min at 100 °C; 2 CV%, percent coefficient of variation.
