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. 2020 Sep 23;9(10):2154. doi: 10.3390/cells9102154

Figure 5.

Figure 5

(A,C) apoptosis of CD3+ T cells of C57BL/6JRj mice (A, n = 4 experiments in triplicates, stimulus concanavalin A (conA) 1.5 µg/mL, 24 h) and healthy human controls (C, n = 7 experiments in triplicates, stimulus phytohemagglutinin (PHA) 0.5 µg/mL, 72 h). Incubation with control or SP 10 µM (Annexin V/PI, flow cytometry). (B,D) proliferation of CD3+ T cells of C57BL/6JRj mice (B, n = 4 experiments in triplicates, stimulus conA 1.5 µg/mL, 24 h) and healthy human controls (D, n = 7 experiments in triplicates, stimulus PHA 0.5 µg/mL, 72 h). Incubation with control or SP 10 µM (CFSE, flow cytometry). Abbreviations: SEM: Standard error of the mean and SP: SP600125. Statistic: Wilcoxon signed rank test # < 0.05, ## < 0.01 and ### < 0.001.