Figure 4.

Shedding of syndecan-1 ectodomain. The amount of shed (soluble) syndecan-1 ectodomain was measured from cell culture media (CCM) by ectodomain-specific antibody. Shedding of the ectodomain was increased in full-length transfectants, whereas it remained unchanged in truncated transfectants in spite of the overall increased abundance of endogenous syndecan-1 (see Figure 3B). Data points represent the mean ± SD, n = 2; * p < 0.05; *** p < 0.001 versus the empty vector transfectant; ^# p < 0.05; ^## p < 0.01 versus the full-length sdc-1 transfectant.