Figure 3.

BSH-polyR directly interacted with the CD44 cell-surface molecule. (A) The immunoprecipitation experiment was performed with a mixture of the cell lysate of MDA-MB-231 and BSH compounds. Anti-BSH antibody successfully precipitated the CD44 molecule from BSH-11R-added samples, while it failed to precipitate CD44 from the BSH-added samples. (B) The ¹⁰B concentration of immunoprecipitates was measured using inductively coupled plasma-atomic emission spectrometry (ICP-AES). The cell lysate of MDA-MB-231 was mixed with BSH compounds and immunoprecipitated with anti-CD44 antibody. (C) The Duolink assay identified the in situ interaction of BSH-11R and CD44.