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. 2020 Sep 23;12(10):2728. doi: 10.3390/cancers12102728

Figure 5.

Figure 5

F. nucleatum accelerates the progression of CRC in AOM/DSS model mice. Tumors were induced in C57BL/6 mice with AOM/DSS. (A) Experimental procedure for the establishment of the ulcerative colitis-related colorectal cancer model and treatment. Abbreviations: i.p. injection, intraperitoneal injection. (B) Body weight changes in mice were monitored at the indicated times after AOM injection. The data are presented as the mean ± SEM (* p < 0.05, and ** p < 0.01; unpaired Student’s t-test). (C) Representative morphological features of the whole colons from mice with or without F. nucleatum infection in the AOM/DSS model (upper panel). Lower panels are enlarged images. (D) Colon length was measured on day 85. The data are presented as the mean ± SEM (* p < 0.05 and *** p < 0.001; unpaired Student’s t-test). (E) The number of tumor nodules in colon tissue was counted on week 12. Data are presented as the average tumor number ± SEM (n = 6). * p < 0.05 compared with the AOM/DSS group. (F) Representative hematoxylin and eosin-stained sections of the distal colon showing the morphology of AOM, AOM/DSS, AOM + F. nucleatum, or AOM/DSS + F. nucleatum-treated mice (n = 5 per group). 100 times magnification; scale bar: 100 μm. Abbreviations: AOM, azoxymethane; DSS, dextran sodium sulfate; Fn, F. nucleatum.