Figure 7.

F. nucleatum promotes EMT through the EGFR signaling pathway. (A) The expression of EGFR pathway-related markers in CRC cells co-cultured with vehicle, DSS, F. nucleatum, or F. nucleatum + DSS was analyzed using Western blotting. Uncropped Western Blot Figures are available in Figure S8. (B) Quantitation of (A). Band intensities of each target protein were measured using an image analyzer and expressed as relative ratios. Data are the mean ± SEM. * p < 0.05 and *** p < 0.005 vs. corresponding controls. (C) The expression of EGFR pathway-related markers and EMT-related markers in CRC cells with or without AG1478 was analyzed using Western blotting. Uncropped Western Blot Figures are available in Figure S9. (D) Quantitation of (C). The band intensities of each target protein were measured using Image J software (ImageJ bundled with 64-bit Java 1.8.0_172, web site: https://imagej.nih.gov/ij/index.html) and expressed as relative ratios. (E) The graph represents relative protein levels obtained from three independent experiments. Densitometry of blots was followed by the calculation of relative protein levels compared with the control after normalization to beta-actin levels. Data are the mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.005 vs. corresponding controls. Abbreviations: AOM, azoxymethane; DSS, dextran sodium sulfate; Fn, F. nucleatum.