Table 2.
Ingredient and calculated nutrient composition of experimental diets, as-fed basis.
| Purified trypsin inhibitor, TIU/g: |
0 |
9,000 |
||
|---|---|---|---|---|
| Protease, PROT/kg: | 0 | 15,000 | 0 | 15,000 |
| Ingredients, g/kg | ||||
| Corn | 562.6 | 552.6 | 512.6 | 502.6 |
| Soybean meal | 345.0 | 345.0 | 345.0 | 345.0 |
| Soybean oil | 18.0 | 18.0 | 18.0 | 18.0 |
| Monocalcium phosphate1 | 11.0 | 11.0 | 11.0 | 11.0 |
| Limestone2 | 13.5 | 13.5 | 13.5 | 13.5 |
| Salt | 2.8 | 2.8 | 2.8 | 2.8 |
| Vitamin-mineral premix3 | 3.0 | 3.0 | 3.0 | 3.0 |
| DL-Methionine | 2.1 | 2.1 | 2.1 | 2.1 |
| L-Lysine HCl | 2.2 | 2.2 | 2.2 | 2.2 |
| Threonine | 1.5 | 1.5 | 1.5 | 1.5 |
| Tryptophan | 0.3 | 0.3 | 0.3 | 0.3 |
| NaHCO3 | 3.0 | 3.0 | 3.0 | 3.0 |
| Trypsin inhibitor premix4 | 0.0 | 0.0 | 50.0 | 50.0 |
| Ronozyme ProAct premix5 | 0.0 | 10.0 | 0.0 | 10.0 |
| Ronozyme HiPhos premix6 | 10.0 | 10.0 | 10.0 | 10.0 |
| Titanium dioxide premix7 | 25.0 | 25.0 | 25.0 | 25.0 |
| Total | 1,000 | 1,000 | 1,000 | 1,000 |
| Calculated composition | ||||
| Crude protein, g/kg | 222.34 | 222.34 | 222.34 | 222.34 |
| ME, kcal/kg | 3,000 | 3,000 | 3,000 | 3,000 |
| Ca, g/kg | 7.95 | 7.95 | 7.95 | 7.95 |
| P, g/kg | 6.11 | 6.11 | 6.11 | 6.11 |
| Nonphytate P, g/kg | 3.54 | 3.54 | 3.54 | 3.54 |
| Ca:total P | 1.30 | 1.30 | 1.30 | 1.30 |
| Na, g/kg | 2.34 | 2.34 | 2.34 | 2.34 |
| K, g/kg | 9.66 | 9.66 | 9.66 | 9.66 |
| Cl, g/kg | 3.72 | 3.72 | 3.72 | 3.72 |
| Dietary electrolyte balance, mEq/kg | 244.49 | 244.49 | 244.49 | 244.49 |
| Digestible amino acids, g/kg | ||||
| Arg | 13.23 | 13.23 | 13.23 | 13.23 |
| His | 5.20 | 5.20 | 5.20 | 5.20 |
| Ile | 8.34 | 8.34 | 8.34 | 8.34 |
| Leu | 17.38 | 17.38 | 17.38 | 17.38 |
| Lys | 12.24 | 12.24 | 12.24 | 12.24 |
| Met | 5.19 | 5.19 | 5.19 | 5.19 |
| Cys | 3.81 | 3.81 | 3.81 | 3.81 |
| Phe | 9.49 | 9.49 | 9.49 | 9.49 |
| Tyr | 7.36 | 7.36 | 7.36 | 7.36 |
| Thr | 8.61 | 8.61 | 8.61 | 8.61 |
| Trp | 2.89 | 2.89 | 2.89 | 2.89 |
| Val | 9.04 | 9.04 | 9.04 | 9.04 |
| Total sulfur amino acids | 9.00 | 9.00 | 9.00 | 9.00 |
| Phe + Tyr | 16.85 | 16.85 | 16.85 | 16.85 |
| Analyzed composition | ||||
| Crude protein, g/kg | 232.1 | 233.0 | 237.8 | 238.6 |
| Crude fiber, g/kg | 24.7 | 25.2 | 25.2 | 25.0 |
| Ether extract, g/kg | 36.5 | 35.7 | 36.2 | 34.1 |
| Protease, PROT/kg8 | LOD | 16,760 | LOD | 17,010 |
| Trypsin inhibitor, TIU/g | 1,181 | 1,218 | 8,882 | 8,833 |
16% Ca, 21% P.
38% Ca.
Supplied the following per kg diet: vitamin A, 5,484 IU; vitamin D3, 2,643 ICU; vitamin E, 11 IU; menadione sodium bisulfite,4.38 mg; riboflavin, 5.49 mg; pantothenic acid, 11 mg; niacin, 44.1 mg; choline chloride, 771 mg; vitamin B12, 13.2 ug; biotin, 55.2 ug; thiamine mononitrate, 2.2 mg; folic acid, 990 ug; pyridoxine hydrochloride, 3.3 mg; I, 1.11 mg; Mn, 66.06 mg; Cu, 4.44 mg; Fe, 44.1 mg; Zn, 44.1 mg; Se, 300 ug.
Purified trypsin inhibitor (PTI) from soybeans product contains 9,000,000 TIU/g. 1 g of PTI added to 49 g of corn supplied 180,000 TIU/g of premix. 50 g premix delivered 9,000,000 TIU/kg feed.
Product contained 75,000 PROT/g. 1 g Protease added to 49 g ground corn supplied 1,500 PROT/g premix. 10 g premix delivered 15,000 PROT/kg feed.
Phytase product contained 5,000 units/g. 1 g of phytase added to 49 g of ground corn supplied 100 units/g of premix. 10 g premix delivered 1,000 units/kg feed. 1,000 units/kg supplied 1.5 g P/kg and 1.7 g of Ca/kg.
1 g of Titanium dioxide added to 4 g of corn.
LOD = limit of detection.