Table 3.
Primers used in real-time quantitative PCR.
| Genes | Primer sequence (5′to 3′) | Gene Bank ID | Reference |
|---|---|---|---|
| Housekeeping gene | |||
| GAPDH | F: TCCTAGGATACACAGAGGACCA | ENSGALG000000144421 | Grenier et al., 2015 |
| R: CGGTTGCTATATCCAAACTCA | |||
| Markers of inflammation | |||
| IL-1β | F: GCATCAAGGGCTACAAGCTC | NM_204524 | Adedokun et al., 2012 |
| R: CAGGCGGTAGAAGATGAAGC | |||
| IL-8 | F: GCGGCCCCCACTGCAAGAAT | ENSGALG000000116701 | Grenier et al., 2015 |
| R: TCACAGTGGTGCATCAGAATTGAGC | |||
| IL-10 | F: GCTGAGGGTGAAGTTTGAGG | ENSGALG000000008921 | Grenier et al., 2015 |
| R: AGACTGGCAGCCAAAGGTC | |||
| Marker of gut integrity | |||
| MUC-2 | F: GCTACAGGATCTGCCTTTGC | XM_421035 | Adedokun et al., 2012 |
| R: AATGGGCCCTCTGAGTTTTT | |||
| Markers of nutrient transport | |||
| ASCT-1 | F: TTGGCCGGGAAGGAGAAG | XM_001232899.4 | Paris and Wong (2013) |
| R: AGACCATAGTTGCCTCATTGAATG | |||
| SLC7A-2 | F: TGCTCGCGTTCCCAAGA | NM_001199102.1 | Gilbert et al. (2007). |
| R: GGCCCACAGTTCACCAACAG | |||
Abbreviations: ASCT-1, neutral amino acid transporter-1; F, forward primer; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL, interleukin; MUC2, mucin 2; R, reverse primer; SLC7A-2, cationic amino acid transporter-2.
1
Sequence obtained from Ensembl chicken genome data resources.