Table 1.

Primer sequences for real-time PCR.

Gene	Primers	Sequence (5′-3′)
Housekeeping	gdhA	Forward 5′-GGAATTGATGTGGCGTTAG-3′
		Reverse 5′-GTGTTGCACATGGTAACG-3′
Endocarditis and biofilm-associated Ebp pilus subunits	ebpA	Forward: 5′-CTACGACACTCTTGCTGGAA-3′ Reverse: 5′-AATTTCGCTCCTTTAAGTGG-3′
	ebpB	Forward: 5′-AACAGAAATTCCTTTTACGG-3′
		Reverse: 5′-ATAGCCAAATGACTACCAGC-3′
	ebpC	Forward: 5′-TTGAAGTGGTGACAGGTGGG-3′
		Reverse: 5′-GCTGCTTTCGTTGTTTCATC-3′
Epa polysaccharide synthesis	epaA	Forward: 5′-TTGCATCACCGCTTGTTATC-3′ Reverse: 5′-TCGCCAACTAGACCGATTAG-3′
	epaB	Forward: 5′-CGGATACAGAAACAACGGAT-3′ Reverse: 5′-TAGAGAATCCGATAGCCTGC-3′
	epaE	Forward: 5′-CGAAGTCAAATTACGCAGTG-3′
		Reverse: 5′-AGGATTCGTGTGTGCCTGTA-3′
	epaG	Forward: 5′-CCGATGGTGAAGAAGACAAT-3′ Reverse: 5′-TGATTGTTTCTGCCAAGCCT-3′
	epaH	Forward: 5′-TCCAAGAAATCCTGGACGAC-3′ Reverse: 5′-CAAATGCCATAAATTCGGCT-3′
	epaM	Forward: 5′-GAGATACGATGGACGTGACT-3′ Reverse: 5′-TGCTTTACCATTTCTAAGGG-3′
	epaQ	Forward: 5′-TGGTTTTGTCGGAGTAGCTG-3′ Reverse: 5′-CCACAAAAGGTTCCATACCG-3′
	epaR	Forward: 5′-AATTTAG`AACGATGTCGGCA-3′ Reverse: 5′-CCGACAATGGACATATCTCC-3′