FIGURE 2.

Bulliform patterning correlates with leaf rolling speed. (a‐d) Grayscale images of leaf epidermal glue‐impressions from four maize inbred lines showing divergent bulliform cell patterning phenotypes: M37W and NC358 have narrow bulliform strips, while B73 and Ki3 have wide strips; M37W and B73 have closely spaced strips (7 per field of view), while NC358 and Ki3 have widely‐spaced strips (4 per field). Arrow = single bulliform strip, arrowhead = macrohair. Scale bar = 500 µm. (e) Distribution of leaf rolling speeds in minutes shown on Y‐axis, after onset of dehydration when 50% rolled status was observed, for 291 inbreds of the WiDiv collection (data can be found in Table S1). Fast‐ and never‐rolling lines used for analysis in F‐H are highlighted. (f‐h) Comparison of bulliform strip number per field of view at standard magnification (f), individual strip width (g), and total bulliform strip width per image (strip number x width) (h). Means in (f‐h) are compared for 25 fast‐rolling maize inbreds (rolled within 90 min) and 38 never‐rolling inbreds (not rolled within 270 min of dehydration), assessed in a detached leaf dehydration assay in the dark in controlled conditions of 20–22°C and 55%–65% humidity. Values are given as means + standard deviation (SD), statistical analysis used two‐tailed unpaired Student's t‐test, with *p < .05, **p < .01