HI titers induced by NDV vaccines. Broilers (n = 12/group) were s.c. (A) or i.m. (B) injected with NDV antigen (Ag) in saline or emulsified in E515-D, Marcol 52, or #10 white oil. Birds injected with saline alone served as a control. Blood samples were collected at 1, 2, 3, 4, 5, and 6 wk after immunization and used for analysis of NDV-specific HI titer. Data are represented as mean ± SD. Bars with different letters are statistically different (P < 0.05). Abbreviations: HI, hemagglutination inhibition; i.m., intramuscularly; NDV, Newcastle disease virus; s.c., subcutaneously.