Table 2.
Primers, sequences, and product size in the PCRs used for detection of resistance genes.
| PCR | Gene | Primer sequence (5′-3′) | Product | Reference |
|---|---|---|---|---|
| 1 | blaTEM | F-TTAACTGGCGAACTACTTAC R- GTCTATTTCGTTCATCCATA |
247 pb | Kozak et al. (2009) |
| blaSHV | F-AGGATTGACTGCCTTTTTG R-ATTTGCTGATTTCGCTCG |
393 pb | Colom et al. (2003) | |
| blaCMY-2 | F- GACAGCCTCTTTCTCCACA R-TGGACACGAAGGCTACGTA |
1,000 pb | Kozak et al. (2009) | |
| 2 | tetA | F- GGCGGTCTTCTTCATCATGC R- GGCAGGCAGAGCAAGTAGA |
502 pb | Lanz et al. (2003) |
| tetB | F- CGCCCAGTGCTGTTGTTGTC R- GCGTTGAGAAGCTGAGGTG |
173 pb | Goswami et al. (2008) | |
| tetC | F- GCTGTAGGCATAGGCTTGGT R- CCGGAAGCGAGAAGAATCA |
888 pb | Lanz et al. (2003) | |
| 3 | ermB-2 | F-GATACC GTTTACGAAATTGG R-GAATCGAGACTTGAGTGTGC |
364 pb | Chen et al. (2007) |
| 4 | qnrS | F- GACGTGCTAACTTGCGTGAT R-TGGCATTGTTGGAAACTTG |
120 pb | Marti and Balcázar (2013) |