Table 2.

Validation of the cubic models of DH and ACE inhibition within the design space.

Parameters			DH (%)1		ACE inhibition (%)1
Temperature (°C)	Enzyme (%)	Time (h)	Actual	Predict	Actual	Predict
50	4	6	34.55	34.32	37.01	36.31
50.31	4	6	34.64	34.42	36.85	36.38
50	3.77	6	33.96	34.16	35.84	36.03
51.07	4	6	35.83	34.58	37.70	36.50
50	2.78	6	32.33	32.58	33.77	34.09
52.22	3.95	5.99	34.82	34.53	36.15	36.33
51.81	3.97	5.95	34.91	34.56	36.51	36.36

Actual values are expressed as mean (n = 3).

Bold indicates the optimized hydrolysis condition obtained from RSM.

Abbreviations: ACE, angiotensin I-converting enzyme; DH, degree of hydrolysis; RSM, response surface methodology.

¹Actual and predict value are not different (P > 0.05).