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. 2020 Oct 30;15(10):e0232568. doi: 10.1371/journal.pone.0232568

Table 6. Species and biovar differentiation of the Brucella isolates.

Brucella isolates Source Growth characteristics Monospecific sera Phage typing Interpretation
Urea H2S CO2 BF TH A M Tb Wb BK2 Fi Iz R/C B. melitensis biovar 3
DXY1 Fetal spleen ++ - - + + + + NL NL CL NL PL NL B. melitensis biovar 3
DXY3 Fetal liver ++ - - + + + + NL NL CL NL PL NL B. melitensis biovar 3
DXY6 Milk ++ - - + + + + NL NL CL NL PL NL B. melitensis biovar 3
DXY8 Ewe’s milk ++ - - + + + + NL NL CL NL PL NL B. melitensis biovar 3
DXY5794 Stomach content ++ - - + + + + NL NL CL NL PL NL B. melitensis biovar 3
DXY1954 Stomach content ++ - - + + + + NL NL CL NL PL NL B. melitensis biovar 3

BF: basic fuchsin at 20 μL/mL (1/50,000 w/v), TH: thionin at 20 μL/mL (1/50,000 w/v), CL: confluent lysis, PL: partial lysis, NL: no lysis.