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(A) WT, (B) M1, (C) M2, (D) M3, (E) M4, (F) M5, (G) M6, (H) M7, (I) M8, and (J) M9: The red and blue contours indicate the −1 and +1 kBT/e isosurface potentials. (K) Connolly surface (transparent gray color) with a CPK model (red color) of five negatively charged amino acids: light chain (LC) Glu27, LC Asp28, LC Asp93, LC Asp56, and heavy chain (HC) Asp31.
