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. 2020 Oct 29;288:114007. doi: 10.1016/j.jviromet.2020.114007

Fig. 1.

Fig. 1

End-point RT PCR for SARS-CoV-2 E gene. A) M: Ladder 100bp marker (Ludwig Biotec, Rio Grande do Sul, Brazil); 1: negative control (water); 2-7 positive control at 10−4 (11.34 vRNA/μL), 10-4.1 (9 vRNA/μL), 10-4.2 (7.15 vRNA/μL), 10-4.3 (5.68 vRNA/μL), 10-5 (1.13 vRNA/μL) and 10-6 (0.11 vRNA/μL), respectively. B) M: Ladder 100bp marker (Ludwig Biotec, Rio Grande do Sul, Brazil); 1: negative sample; 2: positive sample (Ct 34.92). *Interval considered to define the end-point RT-PCR analytical sensitivity.