Table 3.
Primer sequences used for qPCR
| Genes | GeneBank accession no. | Sequence 5′–3′ | Amplicon, bp |
|---|---|---|---|
| CCNB1 | NM_001045872.1 | F: ACACCTACACCAAGTTTCAAATCA R: ATCGTAGTCCAGCATAGTTAGTTCC |
182 |
| CDK1 | NM_174016.2 | F: GGGTCAGCTGGCTACTCAAC R: AGTGCCCAAAGCTCTGAAAA |
138 |
| BCL2 | NM_001166486.1 | F: TGGAGGAGCTCTTCAGGGAC R: GTACTCGGTCATCCACAGGG |
140 |
| BAX | NM_011066.3 | F: GCGCATCGGAGATGAATTGG R: TAGAAAAGGGCGACAACCCG |
153 |
| P53 | NM_174201.2 | F: GAGCACTGCCTACCAACA R: CATCCAGAGCATCCTTCA |
150 |
| CDC25C | NM_001097996.2 | F: TCAGATGCTGGAGGAAGATTCA R: CCCTGGAACTCCCCTGATAGT |
150 |
| MAP2K1 | NM_001130752.1 | F: AGCCGTCCGGACTTGTTATG R: GCCCACGATATAAGGCGAGT |
122 |
| C-MYC | NM_001046273.2 | F: TCTCCTCTGCTGAGTCCTCC R: TTCTGACCTTTTGGCAGGGG |
164 |
| GAPDH | NM_007475.5 | F: GAGATCCTGCCAACATCAA R:CTTCTCCATGGTAGTGAAGAC | 83 |