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. 2020 Sep 24;12(10):2919. doi: 10.3390/nu12102919

Figure 1.

Figure 1

Experimental Animal Model. Female, ~10-week-old, C57Bl/6J mice were fed ad libitum, either a control or a high-fat–high-carbohydrate (HFHC) diet. Age-matched CON and HFHC females were mated once the females in the HFHC group gained 25% of their initial body weight (~14–16 weeks of age). Pregnant dams (CON N = 16, HFHC N = 15) delivered spontaneously. Litters (CON N = 16, HFHC N = 15) were culled to equal size for the lactation period. Animals were maintained on their respective diets throughout gestation and lactation. Pups were weaned at postnatal (PN) day 21 and acclimated to standard chow. Male and female pups were studied at 4–6 weeks of age. control (CON), high-fat–high-carbohydrate (HFHC).