Table 3.
Summary of miRNA biomarker discovery studies in blood cells (in vivo or ex vivo) from TB subjects and controls.
| Subjects No. (Category) |
Controls No. (Category) |
Country | Samples | Method for Screening | Method for Validation | Up-Regulated in TB | Down-Regulated in TB | Ref. | Year |
|---|---|---|---|---|---|---|---|---|---|
| 30 (TB), 35 (LTBI) | 35 HC | China | PBMCs | available microarray dataset | qRT-PCR | miR-212-3p | [109] | 2019 | |
| 3 (TB) | 3 HC | China | PBMCs | small RNA-seq | none | list of 18 miRNAs | list of 23 miRNAs | [110] | 2018 |
| 12 (TB) | 12 HC | South Africa | PBMCs | literature (miRNA selection) | qRT-PCR | miR-320a-3p, miR-204-5p, miR-331-3p, miR-147b, miR-210-3p | miR-197-3p, miR-99b-5p, miR-191-5p | [111] | 2018 |
| 21 (TB) | 21 treated TB | Mexico | PBMCs | literature (miRNA selection) | qRT-PCR | miR-29a-3p, miR-326 | [112] | 2017 | |
| 9 (TB) | 9 HC | Argentina | PBMCs | literature (miRNA selection) | qRT-PCR | miR-29a-3p, miR-30c-5p, miR-181a-5p, miR-181b-5p | [113] | 2017 | |
| 122 (TB) | 130 HC | China | PBMCs, CSF | literature (miRNA selection) | qRT-PCR | miR-29a-3p | [114] | 2017 | |
| 28 (TB) | 24 HC | China | PBMCs | Microarray (Agilent) | qRT-PCR | miR-29b-3p | miR-1-3p, miR-155-5p, miR-31-5p, miR-146a-5p, miR-10a-5p, miR-125b-5p, miR-150-5p | [115] | 2016 |
| 3 (TB), 4 (LTBI) | 3 HC | Hong Kong-China | Macrophages ex vivo | miRNA PCR panel (TaqMan) | none | miR-16-5p, miR-137, miR-140-3p, miR-193a-3p, miR-501-5p, miR-598 | miR-95 | [116] | 2015 |
| 65 (TB) | 60 HC | China | PBMCs | literature (miRNA selection) | qRT-PCR | miR-31 | [117] | 2015 | |
| 30 (TB), 28 (LTBI) | 30 HC | China | CD4 + T cells | Microarray (Exiqon) | qRT-PCR | miR-451a, miR-340-5p, miR-136-5p, miR-29b-3p | miR-4292 | [118] | 2013 |
| 22 (TB), 14 (LTBI) | 19 HC | Germany | CD4 + T cells | literature (miRNA selection) | qRT-PCR | miR-21-5p, miR-26a-5p, miR-29a-3p, miR-142-3p | [39] | 2013 | |
| 24 (TB) | 20 HC | Argentina | PBMCs | literature (miRNA selection) | qRT-PCR | miR-424-5p | miR-146a-5p | [119] | 2012 |
| 29 (TB), 29 (LTBI) | 18 HC | China | PBMCs | Microarray (Agilent) | qRT-PCR | miR-424-5p, miR-365a-3p (vs HC); miR-424-5p, miR-365a-3p, miR-144-3p, miR-223-3p, miR-451a (vs LTB) | [120] | 2011 | |
| 21 (TB) | 19 HC | China | PBMCs induced with PPD | Microarray (Agilent) | qRT-PCR | miR-155-5p, miR-155-3p | [112] | 2011 |