Figure 7.

Construction of KrPL lacY⁺ mutant strain. (A) Domain organization of Lon protease. The N-domain is involved in substrate recognition and binding; the AAA+ domain contains the ATPase module; the Protease domain is responsible for proteins degradation. S679 and K722 represent the catalytic dyad of the proteolytic domain. Fragments B and B’ encoding sequences were chosen as recombination regions. (B) Schematic representation of pVS-lacY vector. (C) Genetic organization of KrPL lacY⁺ selected mutant.