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. 2020 Sep 24;8(10):1466. doi: 10.3390/microorganisms8101466

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Evaluation of production performance of KrPL lon and lacY⁺ mutant strains. (A) β-galactosidase specific activity (U/mg) in lacY⁺ and lon mutant cells harboring p79C-lacZ, collected after progressive times of induction, in GG medium at 15 °C using different concentrations of inducer. Levels of β-galactosidase activity are expressed as mean ± SD, n = 3. (B) Analysis of the relationship between IPTG concentrations and β-galactosidase specific activity measured after 48 h of expression in lacY⁺ and lon strains.