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. 2020 Sep 24;8(10):1466. doi: 10.3390/microorganisms8101466

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Evaluation of β-galactosidase production in KrPL lacY⁺ strain using lactose as the inducer. β-galactosidase specific activity (U/mg) in lacY⁺ and lon mutant cells harboring p79C-lacZ collected after progressive times of induction in GG medium at 15 °C using 2% (w/v) lactose as inducer. Levels of β-galactosidase activity are expressed as mean ± SD, n = 3. (B) Growth curves of KrPL lacY⁺ harboring p79C-lacZ and pP79 in the presence of 2% (w/v) lactose. The growth was performed at 15 °C in GG medium. The moment of the induction is represented by the intersection of the axes. The measures of optical density are expressed as mean ± SD, n = 2.

(A) Evaluation of β-galactosidase production in KrPL lacY⁺ strain using lactose as the inducer. β-galactosidase specific activity (U/mg) in lacY⁺ and lon mutant cells harboring p79C-lacZ collected after progressive times of induction in GG medium at 15 °C using 2% (w/v) lactose as inducer. Levels of β-galactosidase activity are expressed as mean ± SD, n = 3. (B) Growth curves of KrPL lacY⁺ harboring p79C-lacZ and pP79 in the presence of 2% (w/v) lactose. The growth was performed at 15 °C in GG medium. The moment of the induction is represented by the intersection of the axes. The measures of optical density are expressed as mean ± SD, n = 2.