Table 3.
Destruction effect of different EOs alone and in combination with oxacillin on mature biofilm.
| EO mg/mL | Oxacillin µg/mL | Synergism | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Strains | Essential Oil | sMIC50 a | %Destr. ± SD b | sMIC50 c | % Destr. ± SD d | AB ug/mL e | EO mg/mL f | AB ± EO% Destr. ± SD g | FICI |
| E. faecalis ATCC 29212 | Cinnammonum zeylanicum | 50.4 | 52.5 ± 0.70 | 128 | 66.0 ± 0.19 | 4.0 | 2.5 | 56.6 ± 1.00 | 0.08 |
| S.aureus Ig22 | Cinnammonum zeylanicum | 6.3 | 52.1 ± 0.70 | 64 | 59.1 ± 0.40 | 2.0 | 0.3 | 52.2 ± 0.70 | 0.08 |
| S.epidermidis IG4 | Cinnammonum zeylanicum | 12.6 | 60.0 ± 1.00 | 256 | 64.8 ± 0.09 | 7.7 | 0.6 | 64.3 ± 0.04 | 0.08 |
| S. aureus ATCC 29213 | Cinnammonum zeylanicum | 12.6 | 48.0 ± 1.00 | 256 | 47.8 ± 0.50 | 7.7 | 0.6 | 67.2 ± 0.32 | 0.08 |
| E. faecalis ATCC 29212 | Mentha piperita | 11.3 | 50.0 ± 1.00 | 128 | 66.0 ± 0.18 | 8.0 | 0.6 | 45.2 ± 0.90 | 0.11 |
| S. aureus Ig22 | Mentha piperita | 11.3 | 51.1 ± 0.60 | 64 | 59.1 ± 0.50 | 2.0 | 2.3 | 59.0 ± 0.88 | 0.08 |
| S. epidermidis IG4 | Mentha piperita | 45.5 | 44.8 ± 0.70 | 256 | 64.8 ± 1.00 | 7.7 | 2.3 | 67.6 ± 0.50 | 0.08 |
| S. aureus ATCC 29213 | Mentha piperita | 22.8 | 45.1 ± 1.00 | 256 | 47.8 ± 0.70 | 15.4 | 1.1 | 61.9 ± 1.00 | 0.11 |
| E. faecalis ATCC 29212 | Origanum vulgare | 5.5 | 49.2 ± 0.60 | 128 | 66.0 ± 0.05 | 4.0 | 0.3 | 51.9 ± 1.00 | 0.08 |
| S.aureus Ig22 | Origanum vulgare | 11.0 | 57.3 ± 1.00 | 64 | 59.1 ± 0.39 | 2.0 | 0.6 | 61.7 ± 1.00 | 0.23 |
| S. epidermidis IG4 | Origanum vulgare | 5.8 | 61.9 ± 0.60 | 256 | 64.8 ± 0.60 | 7.7 | 0.3 | 53.3 ± 0.77 | 0.08 |
| S. aureus ATCC 29213 | Origanum vulgare | 5.8 | 56.8 ± 1.00 | 256 | 47.8 ± 1.00 | 15.4 | 0.3 | 52.4 ± 0.35 | 0.11 |
| E. faecalis ATCC 29212 | Thymus vulgaris | 21.7 | 47.8 ± 0.80 | 128 | 66.0 ± 1.00 | 4.0 | 1.1 | 58.1 ± 0.69 | 0.08 |
| S. aureus Ig22 | Thymus vulgaris | 43.5 | 50.5 ± 0.50 | 64 | 59.1 ± 0.13 | 2.0 | 2.2 | 55.4 ± 0.65 | 0.08 |
| S. epidermidis IG4 | Thymus vulgaris | 10.9 | 54.1 ± 0.30 | 256 | 64.8 ± 0.20 | 30.7 | 0.5 | 55.0 ± 0.40 | 0.17 |
| S. aureus ATCC 29213 | Thymus vulgaris | 10.9 | 59.4 ± 0.40 | 256 | 47.8 ± 0.90 | 30.7 | 0.5 | 66.1 ± 1.00 | 0.17 |
a: sMIC50: sessile minimal inhibitory concentration 50; b: % biofilm destruction ± standard deviation; c: concentration of the antibiotic in the combination; d: Concentration of the EO in the combination; e: concentration of antibiotic in the mixture; f: concentration of essential oil in the mixture; g: biofilm combination mixture inhibition rate; FICI: fractional inhibitory concentration; AB: antibiotic; EO: essential oil.