Table 2.

Sequencing accuracy (A) and gaps (G) observed when comparing the overlapping number (N) of MinION barcodes with Illumina references.

	R9.4.1_Fast			R9.4.1_HAC			R10.3_Fast_ST			R10.3_HAC_ST			R10.3_Fast_SR			R10.3_HAC_SR
Barcode	N	G	A (%)	N	G	A (%)	N	G	A (%)	N	G	A (%)	N	G	A (%)	N	G	A (%)
MAFFT Ns-filter	65	198	99.9800	74	99	100.0000	62	202	99.9843	76	31	99.9958	69	233	99.9812	77	33	100.0000
RACON	65	119	99.9801	74	50	100.0000	62	121	99.9480	76	7	100.0000	69	125	99.9673	77	9	99.9834
MAFFT+AA (namino1)	62	0	99.9428	73	1	99.9516	61	0	99.9315	75	0	99.9914	68	4	99.9149	76	0	99.9916
MAFFT+AA (namino2)	62	0	99.9479	73	1	99.9736	61	0	99.9525	75	0	99.9957	68	2	99.9479	76	0	100.0000
MAFFT+AA (namino3)	62	2	99.9791	73	1	99.9780	61	0	99.9524	75	0	99.9957	68	4	99.9668	76	0	100.0000
RACON+AA (namino1)	63	1	99.9387	73	0	99.9780	61	4	99.8735	75	0	99.9914	68	0	99.9339	76	0	99.9620
RACON+AA (namino2)	63	1	99.9488	73	0	99.9912	61	4	99.9101	75	0	100.0000	68	0	99.9479	76	0	99.9831
RACON+AA (namino3)	63	1	99.9589	73	0	99.9912	61	5	99.9204	75	0	100.0000	68	0	99.9525	76	0	99.9831
Consolidated (namino1)	55	0	99.9532	70	0	99.9679	58	0	99.9003	73	0	99.9912	64	0	99.9599	74	0	99.9913
Consolidated (namino2)	55	0	99.9648	70	0	99.9862	58	0	99.9223	73	0	99.9956	64	0	99.9649	74	0	100.0000
Consolidated (namino3)	55	0	99.9707	70	0	99.9862	58	0	99.9333	73	0	99.9956	64	0	99.9648	74	0	100.0000