Figure 3.

Increased lamin B1 levels interfere with the perinuclear actin rim levels. (a) Fluorescence microscope micrographs of non-transfected and over-expressing GFP-fused lamin A or GFP-fused lamin B1 confluent B16–F10 cells induced to migrate in the wound healing assay for 3 h stained for filamentous actin (Phalloidin) and DNA (Hoechst). The edge of the scratch is in the top region of each micrograph. The nuclei in the orange rectangles are magnified on the left side. Scale bar: 20 μm. (b) Quantification of the effect of lamins over-expression on perinuclear actin filaments. In each experiment, 20–40 cells of each transfection were measured for the Phalloidin signal at the nuclear periphery. The average mean intensity was calculated and normalized to non-transfected cells. The average mean intensity in three independent experiments ± s.e. is presented. Statistical significance was evaluated by the Student’s t-test, * p < 0.05, ** p < 0.01.