Table 3:
Reaction recipe for circularization of sgRNA expression vector.
| Sample | Volume (μl) |
|---|---|
| PCR product (10-50 ng) | 1 |
| 2X KLD (kinase, ligase, DpnI) Reaction Buffer | 5 |
| 10X KLD Enzyme Mix | 1 |
| Nuclease-free water | 3 |
| Total | 10 |
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