Table 5:
Colony PCR reaction recipe for screening single Toxoplasma clones.
| Sample | Volume (μl) |
|---|---|
| total Toxoplasma genomic DNA | 1 |
| Forward primer (25 μM) | 0.2 |
| Reverse primer (25 μM) | 0.2 |
| 2x PCR master premix | 5 |
| Nuclease-free water | 3.6 |
| Total | 10 |
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