Table 6:

Thermocycler setting for screening single Toxoplasma clones.

Initial denaturation:	98 °C for 5 min
35 cycles of
Denaturing:	98 °C for 5 s
Annealing:	55 - 62 °C for 5 s
Extension:	72 °C for 20 s per kb
Final extension:	72 °C for 1 min