Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay

. Author manuscript; available in PMC: 2020 Oct 30.

Published in final edited form as: J Vis Exp. 2020 Apr 29;(158):10.3791/60985. doi: 10.3791/60985

Materials

Name	Company	Catalog Number
Agarose gel extraction kit	New England BioLabs	T1020L
BamHI	New England BioLabs	R0316S
Biotek Synergy H1 Hybrid Multi-Mode Microplate Reader	BioTek Instuments
BTX Gemini Twin Waveform Electroporation System	Harvard Apparatus
Chemically competent E. coli cells	New England BioLabs	C29871
CloneAmp HiFi PCR premix	Takara Bio	639298
Coelenterazine h	Prolume	301-10 hCTZ
EcoRV	New England BioLabs	R3195S
Phire Tissue Direct PCR Master Mix	Thermo Scientific	F170L
Plasmid miniprep kit	Zymo Research	D4054
Q5 Site-Directed Mutagenesis kit	New England BioLabs	E0554S
Software
Geneious software for sgRNA design (version: R11)
GraphPad Prism software (8^th version)
SnapGene for molecular cloning (version: 4.2.11)