REAGENT or RESOURCE	SOURCE	IDENTIFIER
Cell Lines
Lewis lung carcinoma (LLC1)	ATCC	Cat#CRL-1642; RRID: CVCL_4358
B16-F10	ATCC	Cat#CRL-6475; RRID: CVCL_0159
B16-F10-Luc	Creative Biogene	Cat#CSC-RR0234
Antibodies
Rat anti-mouse CD11b	Biolegend	Cat#101216; RRID: AB_312799
Rat anti-mouse Sca1 (Ly6-A/E)	Biolegend	Cat#122514; RRID: AB_756199
Mouse Lineage Antibody Cocktail	BD Biosciences	Cat#558074; RRID: AB_1645213
Biotin Mouse Lineage Panel	BD Biosciences	Cat#559971; RRID: AB_10053179
Rat anti-mouse cKit (CD117)	BD Biosciences	Cat#553355; RRID: AB_394806
Rat anti-mouse CD16/CD32	Biolegend	Cat#101332; RRID: AB_2650889
Rat anti-mouse CD34	Thermo Fisher Scientific	Cat#11-0341-82; RRID: AB_465021
Rat anti-mouse CD45	Biolegend	Cat#103132; RRID: AB_893340
Rat anti-mouse CD4	Biolegend	Cat#116006; RRID: 313691
Rat anti-mouse CD8	Biolegend	Cat#100722; RRID: AB_312761
Rat anti-mouse Ly6g	Biolegend	Cat#127624; RRID: AB_10640819
Biotin anti-mouse Ly6g	Biolegend	Cat# 127604; RRID: AB_1186108
Rat anti-mouse anti-Ly6c	Biolegend	Cat#128032; RRID: AB_2562178
Biotin anti-mouse Ly6c	Biolegend	Cat#128004; RRID: AB_1236553
Rat anti-mouse F4/80	Biolegend	Cat#123109 and 123116; RRID: AB_893498 and 893481
Rat anti-mouse Gr-1	Biolegend	Cat#108445; RRID: AB_2562903
Rat anti-mouse CD115	Biolegend	Cat#135523; RRID: AB_2566459
Rat anti-mouse CD169	Biolegend	Cat#142417; RRID: AB_2565640
Rat anti-mouse B220	Biolegend	Cat#103207; RRID: AB_312992
Armenian hamster anti-mouse CD11c	Biolegend	Cat#117310; RRID: AB_313779
Rat anti-mouse CD317	Biolegend	Cat#127023; RRID: AB_2687109
Rat anti-mouse CD90.2	Biolegend	Cat#105319; RRID: AB_493724
Rat anti-mouse CD19	Biolegend	Cat#115527; RRID: AB_493734
Mouse anti-mouse NK1.1	Biolegend	Cat#108729; RRID: AB_2074426
Rat anti-mouse anti-IFNα	R&D Systems	Cat#22100-3; RRID: AB_884210
Rat anti-mouse IFNγ	Biolegend	Cat#505808; RRID: AB_315402
Ultra-LEAF Purified anti-mouse IFNAR-1 Antibody	Biolegend	Cat#127321; RRID: AB_11150409
Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl Antibody	Biolegend	Cat#400166; RRID:AB_11146992
Chemicals, Enzymes, and Buffers
Beta-glucan peptide (BGP)	Invivogen	Cat#tlrl-bgp
PMA	Sigma	Cat#P1585
Ionomycin	Sigma	Cat#I0634
Brefeldin A	Biolegend	Cat#420601
Deoxyribonuclease I from bovine pancreas	Sigma-Aldrich	Cat#D5025
Collagenase D	Roche	Cat#11088866001
10X RBC Lysis Buffer	eBioscience	Cat#00-4300-54
Puromycin	Invivogen	Cat#ant-pr1-1
N-Acetyl-L-cysteine	Sigma	Cat#A7250
Nonidet P40 Substrate	Sigma	Cat#74385
Nuclei Buffer	10x Genomics	Cat#2000153
Dynabeads MyOne SILANE	10x Genomics	Cat#2000048
SPRIselect	Beckman Coulter	Cat#B23318
Critical Commercial Assays
CellROX™ Green Flow Cytometry Assay Kit	Thermo Fisher	Cat#C10492
Foxp3/Transcription Factor Buffer Set	eBioscience	Cat#00-5523-00
Intracellular Fixation & Permeabilization Buffer	eBioscience	Cat#88-8824-00
iScript cDNA Synthesis Kit	BioRad	Cat#1708891
High-Capacity RNA-to-cDNA Kit	Applied Biosystems	Cat#4387406
SsoFast EvaGreen Supermix	BioRad	Cat#1725202
Fast SYBR Green Master Mix	Applied Biosystems	Cat#4385612
Anti-Biotin MicroBeads	Miltenyi Biotec	Cat#130-090-485
RNeasy Plus Micro Kit	QIAGEN	Cat#74034
MycoAlertTM Mycoplasma Detection Kit	Lonza	Cat#LT07-318
SMARTer Ultra Low Input RNA for Illumina Sequencing	Takara Bio	Cat#634828
NEBNext Ultra DNA Library Prep Kit for Illumina	New England Biolabs	Cat#E7370L
Luciferase assay system	Promega	Cat#E1500
Chromium Next GEM Chip H Single Cell Kit v1.1	10x Genomics	Cat#1000162
Chromium Next GEM Single Cell ATAC Library & Gel Bead Kit v1.1	10x Genomics	Cat#1000176
NGS High Sensitivity Fragment Analysis Kit	Agilent	Cat#DNF-474
NextSeq 500/550 High Output Kit v2.5;150 Cycles	Illumina	Cat#20024907
Deposited Data
RNA sequencing data	This paper	GEO: GSE139450, GSE139451, GSE139452, and GSE139456
Single cell ATAC sequencing data	This paper	GEO: GSE152353
Experimental models: Organisms/Strains
Mouse: C57BL/6	Janvier Labs	C57BL/6JRj
Mouse: C57BL/6	The Jackson Laboratory	Stock#000664
Mouse: C57BL/6-CD45.1 B6.SJL-PtprcaPepcb/BoyJ	The Jackson Laboratory	Stock#002014
Mouse: B6(Cg)-Ncf1m1J/J	The Jackson Laboratory	Stock#004742
Mouse: B6.129S7-Rag1tm1Mom/J	The Jackson Laboratory	Stock#002216
Mouse: B6(Cg)-Ifnar1tm1.2Ees/J	The Jackson Laboratory	Stock#028288
Oligonucleotides
qPCR primers	This paper	See Table S5
Software, Algorithms and Protocols
GraphPad Prism 6	Graphpad Software	N/A
Ingenuity Pathway Analysis	QIAGEN	https://www.qiagenbioinformatics.com/products/ingenuity-pathway-analysis/
GSEA software	Broad Institute	http://software.broadinstitute.org/gsea/index.jsp
Morpheus	Broad Institute	https://software.broadinstitute.org/morpheus/
DESeq2	Love et al., 2014	https://bioconductor.org/packages/release/bioc/html/DESeq2.html
GSNAP	Wu and Nacu, 2010	http://research-pub.gene.com/gmap/
Ensembl gene annotation version 81	EMBL-EBI	https://www.ebi.ac.uk/
Ensembl Gene IDs using Ensembl Biomart (Release 96)	EMBL-EBI	https://www.ebi.ac.uk/
featureCounts	Liao et al., 2014	http://bioinf.wehi.edu.au/featureCounts/
FlowJo version 10	Tree Star	https://www.flowjo.com/solutions/flowjo
NovoExpress® software	ACEA Biosciences	https://www.aceabio.com
Cell Ranger ATAC version 1.2.0	10x Genomics	https://support.10xgenomics.com/single-cell-atac/software/overview/welcome
R package ChIPseeker v1.22.1	Bioconductor	https://bioconductor.org/packages/release/bioc/html/ChIPseeker.html
R package clusterProfiler v3.14.3	Bioconductor	https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html
Chromium Single Cell ATAC Reagent Kits protocol	10xGenomics	Cat#CG000168