Figure 1.

Mtb-i.v. Induces HSC Expansion and Suppresses Myelopoiesis

(A) The i.v. model.

(B, C, and H–R) WT mice were challenged i.v. with Mtb, Mtb-ΔRD1, or BCG.

(B) Survival (n = 6–8 mice/group).

(C) BM CFUs (n = 4–10 mice/group).

(D–G) In vitro infection of BM cells with H37Rv- or BCG-GFP (4 h, MOI 3). Shown are ImageStream (D) and flow cytometry (F) analysis of infected cells, as a percentage of parental cells in (E) and (G).

(H) Fluorescence-activated cell sorting (FACS) plots and quantification of LKS cells (n = 4–8 mice/group).

(I) Percentage of Ki67⁺ LKS cells on day 7.

(J–L) Frequencies (top) and totals (bottom) of CMPs (J), GMPs (K), and CLPs (L) (n = 4–13 mice/group).

(M–R) Total BM LKS cells (M); LT-HSCs, ST-HSCs, MPPs, CMPs, GMPs, and CLPs (N); MDPs (O); cMoPs (P); GPs (Q); and Ly6C^hi monocytes (R) on day 28.

Log-rank test (B), two-way ANOVA followed by Sidak’s multiple comparisons test (C, H, and J–L), one-way ANOVA followed by Tukey’s multiple comparisons test (I and M), and two-tailed Student’s t test (N–R) were used. Data are representative of two (C, I, and O–R) or three (H and J–N) independent experiments. See also Figure S1.