Figure 4.

Mtb Suppresses Myelopoiesis and Impairs Innate Training in an IFN-I-Dependent Manner

(A and B) Survival following (A) aerosol (n = 6–7 mice/group) or (B) i.v. (n = 8–9 mice/group) infection.

(C–E) Total CMPs (C), GMPs (D), and CLPs (E) after i.v. infection.

(F) Relative BMDM yield.

(G) In vitro BMDM CFUs with Abx in culture.

(H) In vitro BMDM CFUs with Abx in vivo.

(I) Poly(I:C) model.

(J and K) Representative FACS plots of LKS cells as quantified in (K) with percentages (left) and totals (right).

(L–P) Total LT-HSCs (L), ST-HSCs (M), MPPs (N), CMPs (O), and CLPs (P).

(Q) In vitro BMDM CFUs.

(R) β-Glucan model.

(S) In vitro BMDM CFUs (n = 6–7 replicates/group).

(T) Aerosol survival (7 mice/group).

In (A), (B), and (T), log rank test was used. In (C)–(E), (G), (H), (K)–(Q), and (S), two-way ANOVA followed by Sidak’s multiple comparisons test was used. In (F), one-way ANOVA followed by Tukey’s multiple comparisons test was used. Data are representative of two (S) or three (C–Q) independent experiments. See also Figure S4.