Figure 5.

IFN-I Regulates Fe Metabolism in Myeloid Progenitors and Triggers Necrosis

(A) Frequencies of necrotic (NucSpot⁺ AnnexinV⁻) CMPs (left) and GMPs (right) on day 7.

(B) Ripk3 expression in HSCs.

(C–H) Mice were infected i.v. for 7 days.

(C–E) Percentage (left) and total (right) of CMPs (C) and GMPs (D) and total CLPs (E).

(F) CMPs with disrupted MMP.

(G) Relative mitochondrial Fe²⁺ in CMPs.

(H) Representative histograms (left) and quantification (right) of CMP CD71 expression.

(I–L) WT and Ifnar1^−/− mice were infected i.v. for 28 days.

(I) Representative histograms (left) and quantification (right) of CMP CD71 expression.

(J) Percentage of necrotic CMPs.

(K) Relative mitochondrial Fe²⁺ in CMPs.

(L) CMPs with disrupted MMP.

(M–R) WT and Ifnar1^−/− mice were treated with poly(I:C) for 3 days.

(M) CMP CD71 expression.

(N) Representative micrographs (left) and total puncta (right) of BM Perl’s Prussian Blue; the scale bar represents 50 μM.

(O) Relative mitochondrial Fe²⁺ in CMPs.

(P) CMPs with disrupted MMP.

(Q and R) Frequency of ROS⁺ (Q) or necrotic (R) CMPs.

(S–V) WT mice were infected with aerosolized Mtb for 120 days.

(S) Representative histograms (left) and quantification (right) of CMP CD71 expression.

(T) CMPs with disrupted MMP.

(U and V) Percentage of necrotic CMPs (U) or GMPs (V).

In (A) and (F)–(H), one-Way ANOVA followed by Tukey’s comparison test was used. In (C)–(E) and (S)–(V), two-tailed Student’s t test was used. In (I)–(R), two-way ANOVA followed by Sidak’s, Tukey’s, or Dunnett’s multiple comparisons test was used. Data are representative of two (A and F–I) independent experiments or are pooled from 2–3 individual experiments (M–R). See also Figure S5.