Figure 6.

Iron Dysregulation in the BM Promotes Susceptibility to TB

(A) Fth^Δ/Δ model.

(B and C) Percentages (left) and numbers (right) of CMPs (B) or GMPs (C).

(D) BM-Fth^−/− model.

(E) BM ferritin immunoblot; actin = loading control.

(F) Percentage (left) and total (right) of CMPs.

(G and H) Relative mitochondrial Fe²⁺ (G) or disrupted MMP (H) in CMPs.

(I) In vitro BMDM CFUs.

(J and K) BM-Fth^+/+ or BM-Fth^−/− mice were infected with aerosolized Mtb for 28 days. Shown are BM (J) and lung (K) CFUs.

(L) Aerosol survival (n = 5 mice/group).

One-way ANOVA followed by Tukey’s multiple comparisons test was used in (B) and (C). Two-tailed Student’s t test was used in (F)–(H), (J), and (K). Two-way ANOVA followed by Sidak’s multiple comparisons test (I) or log rank test (L) was used. Data are pooled from two experiments (B and C) or representative of two independent experiments (E, F–H, and I). See also Figure S6.