Figure 7.
Mtb-i.v. Causes BM Exhaustion and Has Long-Term Effects on BMDM Training
(A) Mixed chimera model.
(B and C) Percentage of CD45.1+ versus CD45.2+ leukocytes (B) or Ly6Chi monocytes (C) in the blood (n = 5–10/group).
(D–J) In the BM, percentage of LKS cells (D), LT-HSCs (E), ST-HSCs (F), MPPs (G), CMPs (H), GMPs (I), and CLPs (J) after 16 weeks of reconstitution.
(K) Secondary engraftment model.
(L–N) Number of LKS cells (L), LT-HSCs (M), and CMPs (N).
(O and P) Myeloid colonies (O) and cell type by flow cytometry (P) by BM methylcellulose CFU assay.
(Q) In vitro BMDM CFUs.
(R and S) Number of genes up- and downregulated by Mtb and BCG in each cluster (abs(logFC) > 0.1 and FDR < 0.05) of LKS cells (R) and cKit+ cells (S).
(T) GSEA of genes ranked according to BCG (left) or Mtb (right) effects in the GMP_MonP cluster.
(U) Distribution of expression levels in GMP_MonP for gene set average markers in four different hallmark gene sets.
(V) Il6ra and Flt3 expression in the GMP_MonP cluster.
Two-way ANOVA followed by Sidak’s multiple comparisons test was used in (B), (C), (P), and (Q). One-way ANOVA followed by Tukey’s multiple comparisons test was used in (L)–(O). In (D–J), two-tailed Student’s t test was used to assess differences. In (B) and (C), only significant differences at 16 weeks are depicted. See also Figure S7.