Figure S2.

HSC Imprinting by BCG and Mtb-i.v. and Subsequent Anti-mycobacterial Responses by Macrophages to Mtb Infection In Vitro, Related to Figure 2

(A) Scatterplot for significance levels (-log10(p value) of gene ontology enrichment analyses conducted among DE genes upon BCG versus Mtb infections in HSC (left) and MPP (right). (B) Gene ontology terms enriched among DEG in response to Mtb in MPP (at FDR < 0.01). (C-F) BMDM from PBS control, BCG-iv and Mtb-iv groups were generated. Purity of BMDM cultures as determined by flow cytometry using expression of BMDM markers CD11b and F4/80 (C). Activation of mature BMDM was assessed by flow cytometry via MFIs of CD80 (D), CD40 (E) and MHC-II (F) with representative histograms in the top panels. Model of in vivo antibiotic treatment (G). BMDM-derived from the BM cells of these mice were infected with Mtb (H37Rv; MOI 1) and the number of CFU was determined at different time points after infection (H). (I) BMDM CFU prior to intratracheal transfer, as detailed in Figures 2H and 2I. Differences determined by One-way ANOVA followed by Tukey’s Multiple Comparisons Test in C-F and I; Two-way ANOVA followed by Tukey’s Multiple Comparisons Test in H.