Figure S4.

IFN-I Signaling Impairs Myelopoiesis, Related to Figure 4

(A-C) WT and Ifnar1^−/− mice were infected with 1x10⁶Mtb-iv. Kinetics of the frequencies among single viable BM cells of CMP (A), GMP (B) and CLP (C). (D-F) mice were infected with Mtb via the aerosol route. Percentages of CMP (D), GMP (E) and CLP (F) in the BM at day 60. (G) Active IFN-I was measured in the BM collected after 12, 24, and 36 hours after Poly (I:C) treatment. (H-Q) WT and Ifnar1^−/− mice were treated with Poly (I:C), or PBS, at day 0, 2, 4, 6. Frequencies among single viable BM cells of LT-HSC (H), ST-HSC (I), MPP (J), CMP (K) and CLP (L) at days 0, 3 and 7, as well as the total number of MDP (M), cMoP (N), GP (O), Ly6C^hi monocytes (P) and neutrophils (Q) at day 3 post-Poly-(I:C) treatment. (R-X) WT mice were treated with β-glucan at day 0 and day 3. Frequencies among BM cells (left panel) and total cell counts (right panel) of LKS (R), LT-HSC (S), ST-HSC (T), MPP (U), CMP (V), GMP (W) and CLP (X) in PBS (day 0) or β-glucan i.p. at day 7 post-treatment. Differences determined by Two-way ANOVA followed by Sidak’s Multiple Comparisons Test in A-C, H-Q, Two-Tailed Student’s T-Test in D-F, One-way ANOVA followed by Dunnett’s Multiple Comparisons Test in G and Non-Parametric Mann-Whitney Test R-X.