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. 2020 Oct 29;17:324. doi: 10.1186/s12974-020-01982-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — α-Axl antibody treatment has no effect on the number of Iba1⁺ microglia, T cells, T_regs, or axonal damage in the spinal cord during acute EAE. Quantification of Iba1 (a), CD3 (b), FoxP3 (c), and SMI32 (d) immune-stained lumbar spinal cord sections of PBS- (n = 6), IgG isotype control- (n = 6), or α-Axl-activating antibody-treated (n = 6) mice during acute EAE. Quantification of the SMI32⁺ axonal swellings (> 3 μm) was conducted on multiple × 20 fields of the left and the right ventral regions of the lumbar spinal cord. *p ≤ 0.05, Mann-Whitney U test