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. 2020 Oct 30;6:12. doi: 10.1038/s41514-020-00050-7

Fig. 1. Iron induces canonical Wnt signaling in the retinal pigment epithelial cells.

Fig. 1

a Total and active β-catenin (non-phospho) protein levels were estimated by western blot in ARPE19 cells treated with different concentrations of FAC for 24 h. β-Actin was used as a loading control. b Protein expression of GSK-3β, p-GSK-3β, LRP6, and p-LRP6 were determined by western blotting. β-Actin was used as a loading control. c Expression of active β-catenin in cytosolic and nuclear fractions isolated from ARPE19 cells treated with FAC for 24 h. β-Actin and HDAC1 were used as loading controls for cytosolic and nuclear fractions respectively. In a–c, blots cropped from different parts of the same gel or from different gels are separated by white space. d mRNA expression of Wnt downstream target genes Axin2, cMYC, CCND, and VEGF in the cells treated with different concentrations of FAC for 24 h. e Renilla luciferase activity in the TCF/LEF Reporter transfected cells. LiCl and Wnt3a were used as a positive control. Data presented as mean ± SE of three independent experiments; *p < 0.05; **p < 0.001.