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. 2020 Oct 30;6:12. doi: 10.1038/s41514-020-00050-7

Fig. 4. Iron induced Wnt signaling activation is mediated by oxidative stress.

Fig. 4

a, b Expression of Src collagen homolog (SHC1), an indicator of redox imbalance and oxidative stress, in the ARPE19 cells treated with FAC for 24 h and in mice treated with intraperitoneal iron dextran for 8 weeks. c ARPE19 cells when treated with FAC for 24 h along with antioxidant N-acetyl cysteine (NAC) or iron chelator deferiprone (DFP) normalized active β-catenin and LRP6 levels in western blotting. d Cytosolic and nuclear fractions were separated and the expression of active β-catenin was determined by western blotting. β-Actin and HDAC1 were used as loading controls for cytosolic and nuclear fractions respectively. Blots cropped from different parts of the same gel or from different gels are separated by white space. Data presented as mean ± SE of three independent experiments. *p < 0.05; **p < 0.001.