Fig. 5. PPARα agonist fenofibrate prevents iron-induced Wnt signaling activation in ARPE19 cells.

ARPE19 cells were treated with 50 and 100 µM of Fenofibrate (Feno) along with 100 µg/ml FAC for 24 h, and the protein expression of a active and T-β-catenin, b p-LRP6 and p-GSK-3β were determined by immunoblotting. β-Actin was used as a loading control. c Cytosolic and nuclear proteins were isolated after the cells were treated with fenofibrate in the presence of FAC for 24 h and the expression of active β-catenin was determined by western blotting. d Active β-catenin, total β-catenin, and SHC1 protein levels were assessed by western blotting in mouse primary RPE cells treated with FAC and fenofibrate. Blots cropped from different parts of the same gel or from different gels are separated by white space. Data presented as mean ± SE of three independent experiments. *p < 0.05; **p < 0.001.