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. 2020 Oct 30;11:5504. doi: 10.1038/s41467-020-19365-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Sample size N ranges from 40 to 120 individuals in steps of 8 and the number of cells per individuals M ranges from 500 to 2750 cells per individual in steps of 250 (CD4 T cells). a Library preparation is assumed to be 0$ per reaction, level of multiplexing is fixed and equal to 8. b Library preparation is set to $2000 per reaction, level of multiplexing is fixed and equal to 8. c Library preparation is set to $2000 per reaction, greedy multiplexing. d Library preparation is set to $2000 per reaction, greedy multiplexing, demultiplexing inaccuracy, and cell-type misclassification is taken into account.