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. 2020 Oct 30;3:630. doi: 10.1038/s42003-020-01353-x

Fig. 5. Targeting AIMP2-DX2 expression can sensitize cells to anticancer drugs.

Fig. 5

a AIMP2-DX2 protein expression after electroporation with siRNA targeting AIMP2-DX2 mRNA. AIMP2-DX2 protein was detected only in HL-60, but not in Namalwa nor KMS-12-BM cells. In HL-60 cells, siAIMP2-DX2 effectively reduced the AIMP2-DX2 protein expression. TUBB was used as loading control. b–d Knockdown of AIMP2-DX2 enhanced apoptosis induced by paclitaxel, etoposide, and cisplatin in HL-60 cells (b), but not in Namalwa (c) nor KMS-12-BM cells (d). For Namalwa cells, knockdown of AIMP2-DX2 partially rescued the cell death by all drugs. n = 4 for paclitaxel in all three cell lines. n = 3 for HL-60, n = 7 for Namalwa, n = 4 for KMS-12-BM for etoposide. n = 6 for HL-60, n = 9 for Namalwa, n = 9 for KMS-12-BM for cisplatin. A solid red line indicates cell viability relative to siLuc, while a black dashed line indicates cell viability of control. For all data presented, an average is shown with error bars indicate s.e.m. *P < 0.05, **P < 0.01. For statistical analysis, a one-tailed t test with unequal variance was used.