Fig. 3. Growth curves of bladder cell lines transfected with either the minigene circuit or the traditional circuit.

Proliferations of the transfected cells “high grade-1” (a), “high grade-2” (b), “high grade-3”(c), “high grade-4” (d), “low grade-1” (e), “low grade-2” (f), “low grade-3”(g), “low grade-4” (h), “normal-1” (i), “normal-2” (j), and “normal-3” (k) were measured using the CCK-8 assay at different time intervals. The minigene circuit without output gene was used as the negative control. The curves of cell proliferations were compared using two-way analysis of variance (ANOVA). The results at each time point are shown as the mean ± SD. Each experiment was independently performed in triplicate five times. Each error bar indicates the variation between the means of five independent experiments. ^*p < 0.05 and ^**p < 0.01 between the groups. Exact p-values for asterisks (from left to right): 0.0008, 0.0059, 0.0061, 0.0007, 0.0034, 0.0011, 0.0057, 0.0019, 0.0063, 0.0023, 0.0021, 0.0064, 0.0062, 0.0055, 0.0054, 0.0059, 0.0053, 0.034, 0.034, 0.036, 0.036, 0.041, and 0.038. OD optical density. Source data are provided as a Source Data file.